셀투인은 연구개발 결과를 특허등록, 학술지 게재 및 국제학회 기술 발표를 통해
기술의 우수성과 신뢰성을 입증하고 있습니다.
지적재산권 확보
Cell2in은 글로벌 바이오 시장에서 고유 기술을 활용한 적극적 사업 전개를 위해 국내는 물론 신약개발 주요국가의 고품질 세포치료제 생산기술의 특허를 확보하고 있습니다.
물질 및 용도
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
세포의 티올수준을 측정하기 위한 실시간 이미징 센서 |
Korea | 10-1862581 | 2018. 05. 24 |
United States | 10.215.757B2 | 2019. 02. 26 | |
Korea | 10-1983803 | 2019. 05. 23 | |
United States | 10.620.215 | 2020. 04. 14 | |
세포의 소기관 내 글루타치온 측정용 실시간 형광 이미징 센서 및 이의 제조방법 |
Korea | 10-2133794 | 2020. 07. 08 |
Canada (application) | 3.072.434 | 2020. 02. 07 | |
Australia (application) | 2018320601 | 2020. 02. 19 | |
United States (application) | 16/640717 | 2020. 02. 20 | |
Europe (application) | 18848372.1 | 2020. 02. 21 | |
China (application) | 201880054675.0 | 2020. 02. 21 | |
India (application) | 202017007994 | 2020. 02. 25 | |
Japan (application) | 2020-511502 | 2020. 02. 19 | |
소포체 내 글루타치온 측정용 실시간 형광 이미지 센서 및 이를 이용하는 방법 |
Korea | 10-2019-0178260 | 2019. 12. 30 |
Gene Biomarker
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
FreSHtracer 기반 분리된 세포의 유전자 프로파일의 응용 |
PCT | PCT/KR 2018/008239 | 2018. 07. 20 |
Stem Cell Therapy
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
혈소판 감소증 치료용 조성물 |
Korea | 10-2021-0063975 | 2021. 05. 18 |
고품질의 줄기세포 선별용 마커 및 이를 이용한 고품질 줄기세포 선별 방법 |
Korea | 10-2020-0180455 | 2020. 12. 22 |
Cell Quality Control
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
실시간 글루타치온 측정을 통한 치료용 세포의 품질 향상 방법 |
Korea | 10-2119714 | 2020. 06. 01 |
Europe (application) | 18883520.1 | 2020. 05. 27 | |
China (application) | 201880077266.2 | 2020. 05. 28 | |
Japan (application) | 2020-546264 | 2020. 05. 27 | |
United States (application) | 16/767985 | 2020. 05. 28 | |
실시간 글루타치온 측정을 통한 치료용 세포의 품질 측정 방법 |
Korea | 10-2145929 | 2020. 08. 12 |
United States (application) | 16/768014 | 2020. 05. 28 | |
Europe (application) | 18884812.1 | 2020. 05. 27 | |
China (application) | 201880077236.1 | 2020. 05. 28 | |
Japan (application) | 2020-546265 | 2020. 05. 27 |
기술 발표 및 게재
셀투인은 국내외 학회와 학술지를 통해 연구 성과를 지속적으로 발표해오고 있습니다.
SOP of Development Technology : EM. Jeong et al., Monitoring Glutathione Dynamics and Heterogeneity in Living Stem Cells, Int J Stem Cells, (2019) 12(2), 367-379
Glutathione (GSH) is a major antioxidant in cells, and plays vital roles in the cellular defense against oxidants and
in the regulation of redox signals. In a previous report, we demonstrated that stem cell function is critically affected
by heterogeneity and dynamic changes in cellular GSH concentration. Here, we present a detailed protocol for the
monitoring of GSH concentration in living stem cells using FreSHtracer, a real-time GSH probe. We describe the
steps involved in monitoring GSH concentration in single living stem cells using confocal microscopy and flow
cytometry. These methods are simple, rapid, and quantitative, and able to demonstrate intracellular GSH concentration
changes in real time. We also describe the application of FreSHtracer to the sorting of stem cells according to their
GSH content using flow cytometry. Typically, microscopic or flow cytometric analyses of FreSHtracer and
MitoFreSHtracer signals in living stem cells take ∼2∼3 h, and the fractionation of stem cells into subpopulations
on the basis of cellular GSH levels takes 3∼4.5 h. This method could be applied to almost every kind of mammalian
cell with minor modifications to the protocol described here.
Keywords: Glutathione, Real-time monitoring, Fluorescent probes, Stem cells
in the regulation of redox signals. In a previous report, we demonstrated that stem cell function is critically affected
by heterogeneity and dynamic changes in cellular GSH concentration. Here, we present a detailed protocol for the
monitoring of GSH concentration in living stem cells using FreSHtracer, a real-time GSH probe. We describe the
steps involved in monitoring GSH concentration in single living stem cells using confocal microscopy and flow
cytometry. These methods are simple, rapid, and quantitative, and able to demonstrate intracellular GSH concentration
changes in real time. We also describe the application of FreSHtracer to the sorting of stem cells according to their
GSH content using flow cytometry. Typically, microscopic or flow cytometric analyses of FreSHtracer and
MitoFreSHtracer signals in living stem cells take ∼2∼3 h, and the fractionation of stem cells into subpopulations
on the basis of cellular GSH levels takes 3∼4.5 h. This method could be applied to almost every kind of mammalian
cell with minor modifications to the protocol described here.
Keywords: Glutathione, Real-time monitoring, Fluorescent probes, Stem cells
학술지 게재
『Stem Cell Report』 | |
『The Journal of Clinical Investigation』 | |
『Science Advances』 |
기술 발표
국제줄기세포학회 기술 발표 |