在全球生物市场利用特有技术积极开展事业
细胞批量培养技术 + 质量管理技术
兼具两种核心技术的企业
获得主导新药开发市场的
主要国家的专利
利用专利技术开拓国际市场
知识产权
我们通过建立知识产权保护核心技术
技术名称 | 申请国家 | 申请·注册号 | 申请·登记日期 |
---|---|---|---|
用于检测细胞硫醇水平的 实时成像传感器 |
韩国 | 10-1862581 | 2018. 05. 24 |
美国 | 10.215.757B2 | 2019. 02. 26 | |
韩国 | 10-1983803 | 2019. 05. 23 | |
美国 | 10.620.215 | 2020. 04. 14 | |
细胞器内谷胱甘肽检测用 实时荧光成像传感器及其制造方法 |
韩国 | 10-2133794 | 2020. 07. 08 |
加拿大(申请) | 3.072.434 | 2020. 02. 07 | |
澳大利亚(申请) | 2018320601 | 2020. 02. 19 | |
美国(申请) | 16/640717 | 2020. 02. 20 | |
欧洲(申请) | 18848372.1 | 2020. 02. 21 | |
中国(申请) | 201880054675.0 | 2020. 02. 21 | |
印度(申请) | 202017007994 | 2020. 02. 25 | |
日本(申请) | 2020-511502 | 2020. 02. 19 | |
内质网内谷胱甘肽检测用 实时荧光成像传感器及其使用方法 |
韩国 | 10-2019-0178260 | 2019. 12. 30 |
Gene Biomarker
技术名称 | 申请国家 | 申请·注册号 | 申请·登记日期 |
---|---|---|---|
基于 FreSHtracer 分离的细胞 基因配置文件的应用 |
PCT | PCT/KR 2018/008239 | 2018. 07. 20 |
Stem Cell Therapy
技术名称 | 申请国家 | 申请·注册号 | 申请·登记日期 |
---|---|---|---|
用于治疗血小板减少症的组合物 | 韩国 | 10-2021-0063975 | 2021. 05. 18 |
用于评估干细胞特性的 基因标记及其用途 |
韩国 | 10-2020-0180455 | 2020. 12. 22 |
Cell Quality Control
技术名称 | 申请国家 | 申请·注册号 | 申请·登记日期 |
---|---|---|---|
通过实时检测谷胱甘肽, 提高治疗用细胞质量的方法 |
韩国 | 10-2119714 | 2020. 06. 01 |
欧洲(申请) | 18883520.1 | 2020. 05. 27 | |
中国(申请) | 201880077266.2 | 2020. 05. 28 | |
日本(申请) | 2020-546264 | 2020. 05. 27 | |
美国(申请) | 16/767985 | 2020. 05. 28 | |
通过实时检测谷胱甘肽, 检测治疗用细胞质量的方法 |
韩国 | 10-2145929 | 2020. 08. 12 |
美国(申请) | 16/768014 | 2020. 05. 28 | |
欧洲(申请) | 18884812.1 | 2020. 05. 27 | |
中国(申请) | 201880077236.1 | 2020. 05. 28 | |
日本(申请) | 2020-546265 | 2020. 05. 27 |
发表
接连在知名学术期刊上发表了研究成果
SOP of Development Technology : EM. Jeong et al., Monitoring Glutathione Dynamics and Heterogeneity in Living Stem Cells, Int J Stem Cells, (2019) 12(2), 367-379
Glutathione (GSH) is a major antioxidant in cells, and plays vital roles in the cellular defense against oxidants and
in the regulation of redox signals. In a previous report, we demonstrated that stem cell function is critically affected
by heterogeneity and dynamic changes in cellular GSH concentration. Here, we present a detailed protocol for the
monitoring of GSH concentration in living stem cells using FreSHtracer, a real-time GSH probe. We describe the
steps involved in monitoring GSH concentration in single living stem cells using confocal microscopy and flow
cytometry. These methods are simple, rapid, and quantitative, and able to demonstrate intracellular GSH concentration
changes in real time. We also describe the application of FreSHtracer to the sorting of stem cells according to their
GSH content using flow cytometry. Typically, microscopic or flow cytometric analyses of FreSHtracer and
MitoFreSHtracer signals in living stem cells take ∼2∼3 h, and the fractionation of stem cells into subpopulations
on the basis of cellular GSH levels takes 3∼4.5 h. This method could be applied to almost every kind of mammalian
cell with minor modifications to the protocol described here.
Keywords: Glutathione, Real-time monitoring, Fluorescent probes, Stem cells
in the regulation of redox signals. In a previous report, we demonstrated that stem cell function is critically affected
by heterogeneity and dynamic changes in cellular GSH concentration. Here, we present a detailed protocol for the
monitoring of GSH concentration in living stem cells using FreSHtracer, a real-time GSH probe. We describe the
steps involved in monitoring GSH concentration in single living stem cells using confocal microscopy and flow
cytometry. These methods are simple, rapid, and quantitative, and able to demonstrate intracellular GSH concentration
changes in real time. We also describe the application of FreSHtracer to the sorting of stem cells according to their
GSH content using flow cytometry. Typically, microscopic or flow cytometric analyses of FreSHtracer and
MitoFreSHtracer signals in living stem cells take ∼2∼3 h, and the fractionation of stem cells into subpopulations
on the basis of cellular GSH levels takes 3∼4.5 h. This method could be applied to almost every kind of mammalian
cell with minor modifications to the protocol described here.
Keywords: Glutathione, Real-time monitoring, Fluorescent probes, Stem cells