We aim to become the global biotech company
based on proprietary technology.
R&D based company with mass cell culture technology and cell quality management technology
Patented in countries
leading new drug development market
Enter the global biotech market
with Proprietary technology
Intellectual Property
Cell2in is committed to protect our core technology by establishment of intellectual property in the field of cell therapy
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
Real-time Imaging Sensor for Measuring Cellular Thiol Levels | Korea | 10-1862581 | 2018. 05. 24 |
United States | 10.215.757B2 | 2019. 02. 26 | |
Korea | 10-1983803 | 2019. 05. 23 | |
United States | 10.620.215 | 2020. 04. 14 | |
Real-time Fluorescence Imaging Sensor for Estimating the Glutathione in Cellular Organelle and Manufacturing Method Thereof | Korea | 10-2133794 | 2020. 07. 08 |
Canada (application) | 3.072.434 | 2020. 02. 07 | |
Australia (application) | 2018320601 | 2020. 02. 19 | |
United States (application) | 16/640717 | 2020. 02. 20 | |
Europe (application) | 18848372.1 | 2020. 02. 21 | |
China (application) | 201880054675.0 | 2020. 02. 21 | |
India (application) | 202017007994 | 2020. 02. 25 | |
Japan (application) | 2020-511502 | 2020. 02. 19 | |
Real-time Fluorescence Imaging Sensor for Estimating the Glutathione in Endoplasmic Reticulum and a Method of Using It | Korea | 10-2019-0178260 | 2019. 12. 30 |
Gene Biomarker
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
FreSHtracer-based Application of the Genetic Profile of Separated Cells | PCT | PCT/KR 2018/008239 | 2018. 07. 20 |
Stem Cell Therapy
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
Composite for Treatment of Thrombocytopenia | Korea | 10-2021-0063975 | 2021. 05. 18 |
Gene Market for Evaluating the Characteristics of Stem Cells and the Use of the Same | Korea | 10-2020-0180455 | 2020. 12. 22 |
Cell Quality Control
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
Method of Therapeutic Cell Quality Improvement Based on Real-time Glutathione Measurement | Korea | 10-2119714 | 2020. 06. 01 |
Europe (application) | 18883520.1 | 2020. 05. 27 | |
China (application) | 201880077266.2 | 2020. 05. 28 | |
Japan (application) | 2020-546264 | 2020. 05. 27 | |
United States (application) | 16/767985 | 2020. 05. 28 | |
Method of Therapeutic Cell Quality Measurement Based on Real-time Glutathione Measurement | Korea | 10-2145929 | 2020. 08. 12 |
United States (application) | 16/768014 | 2020. 05. 28 | |
Europe (application) | 18884812.1 | 2020. 05. 27 | |
China (application) | 201880077236.1 | 2020. 05. 28 | |
Japan (application) | 2020-546265 | 2020. 05. 27 |
Publication
Cell2in has continuously published its research results through famous academic societies and journals.
SOP of Development Technology : EM. Jeong et al., Monitoring Glutathione Dynamics and Heterogeneity in Living Stem Cells, Int J Stem Cells, (2019) 12(2), 367-379
Glutathione (GSH) is a major antioxidant in cells, and plays vital roles in the cellular defense against oxidants and
in the regulation of redox signals. In a previous report, we demonstrated that stem cell function is critically affected
by heterogeneity and dynamic changes in cellular GSH concentration. Here, we present a detailed protocol for the
monitoring of GSH concentration in living stem cells using FreSHtracer, a real-time GSH probe. We describe the
steps involved in monitoring GSH concentration in single living stem cells using confocal microscopy and flow
cytometry. These methods are simple, rapid, and quantitative, and able to demonstrate intracellular GSH concentration
changes in real time. We also describe the application of FreSHtracer to the sorting of stem cells according to their
GSH content using flow cytometry. Typically, microscopic or flow cytometric analyses of FreSHtracer and
MitoFreSHtracer signals in living stem cells take ∼2∼3 h, and the fractionation of stem cells into subpopulations
on the basis of cellular GSH levels takes 3∼4.5 h. This method could be applied to almost every kind of mammalian
cell with minor modifications to the protocol described here.
Keywords: Glutathione, Real-time monitoring, Fluorescent probes, Stem cells
in the regulation of redox signals. In a previous report, we demonstrated that stem cell function is critically affected
by heterogeneity and dynamic changes in cellular GSH concentration. Here, we present a detailed protocol for the
monitoring of GSH concentration in living stem cells using FreSHtracer, a real-time GSH probe. We describe the
steps involved in monitoring GSH concentration in single living stem cells using confocal microscopy and flow
cytometry. These methods are simple, rapid, and quantitative, and able to demonstrate intracellular GSH concentration
changes in real time. We also describe the application of FreSHtracer to the sorting of stem cells according to their
GSH content using flow cytometry. Typically, microscopic or flow cytometric analyses of FreSHtracer and
MitoFreSHtracer signals in living stem cells take ∼2∼3 h, and the fractionation of stem cells into subpopulations
on the basis of cellular GSH levels takes 3∼4.5 h. This method could be applied to almost every kind of mammalian
cell with minor modifications to the protocol described here.
Keywords: Glutathione, Real-time monitoring, Fluorescent probes, Stem cells