We aim to become the global biotech company
based on proprietary technology.
R&D based company with mass cell culture technology and cell quality management technology
Patented in countries
leading new drug development market
Enter the global biotech market
with Proprietary technology
Intellectual Property
Cell2in is committed to protect our core technology by establishment of intellectual property in the field of cell therapy
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
Real-time Imaging Sensor for Measuring Cellular Thiol Levels | Korea | 10-1862581 | 2018. 05. 24 |
United States | 10.215.757B2 | 2019. 02. 26 | |
Korea | 10-1983803 | 2019. 05. 23 | |
United States | 10.620.215 | 2020. 04. 14 | |
Real-time Fluorescence Imaging Sensor for Estimating the Glutathione in Cellular Organelle and Manufacturing Method Thereof | Korea | 10-2133794 | 2020. 07. 08 |
Canada (application) | 3.072.434 | 2020. 02. 07 | |
Australia (application) | 2018320601 | 2020. 02. 19 | |
United States (application) | 16/640717 | 2020. 02. 20 | |
Europe (application) | 18848372.1 | 2020. 02. 21 | |
China (application) | 201880054675.0 | 2020. 02. 21 | |
India (application) | 202017007994 | 2020. 02. 25 | |
Japan (application) | 2020-511502 | 2020. 02. 19 | |
Real-time Fluorescence Imaging Sensor for Estimating the Glutathione in Endoplasmic Reticulum and a Method of Using It | Korea | 10-2019-0178260 | 2019. 12. 30 |
Gene Biomarker
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
FreSHtracer-based Application of the Genetic Profile of Separated Cells | PCT | PCT/KR 2018/008239 | 2018. 07. 20 |
Stem Cell Therapy
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
Composite for Treatment of Thrombocytopenia | Korea | 10-2021-0063975 | 2021. 05. 18 |
Gene Market for Evaluating the Characteristics of Stem Cells and the Use of the Same | Korea | 10-2020-0180455 | 2020. 12. 22 |
Cell Quality Control
Name of Technology | Country of Application | Application and Registration No. | Applied and Registered Date |
---|---|---|---|
Method of Therapeutic Cell Quality Improvement Based on Real-time Glutathione Measurement | Korea | 10-2119714 | 2020. 06. 01 |
Europe (application) | 18883520.1 | 2020. 05. 27 | |
China (application) | 201880077266.2 | 2020. 05. 28 | |
Japan (application) | 2020-546264 | 2020. 05. 27 | |
United States (application) | 16/767985 | 2020. 05. 28 | |
Method of Therapeutic Cell Quality Measurement Based on Real-time Glutathione Measurement | Korea | 10-2145929 | 2020. 08. 12 |
United States (application) | 16/768014 | 2020. 05. 28 | |
Europe (application) | 18884812.1 | 2020. 05. 27 | |
China (application) | 201880077236.1 | 2020. 05. 28 | |
Japan (application) | 2020-546265 | 2020. 05. 27 |
Publication
Cell2in has continuously published its research results through famous academic societies and journals.
Development Technology and senescent Cells : KB. Lee et al., Antioxidants reduce the heterogeneity of the intracellular glutathione level in senescent cell population of human dermal fibroblasts, J Dermatol Sci. (2020) 98(3),195-198
Accumulating evidence suggests that increased reactive
oxygen species (ROS) production has a role in inducing
senescence of human dermal
fibroblasts (HDFs) via the activation
of DNA damage and redox signaling pathways [1]. Unfortunately,
ROS-reducing antioxidant agents are only marginally effective in
delaying or preventing cellular senescence, even at a high
concentration [2,3]. We evaluated endogenous antioxidant
systems to better understand these
findings. Glutathione (GSH)
is the most abundant thiol-containing tripeptide that plays a
major role in eliminating H2O2 through a GSH peroxidase-
catalyzed reactions [4]. Moreover, GSH reduces oxidized proteins
through glutathionylation, thereby regulating the intensity and
duration of redox signaling. Thus, intracellular GSH level are
critical for maintaining redox homeostasis. Measuring GSH level
in live cells is challenging owing to the irreversible reaction of
fluorescent probes with GSH. We developed a new coumarin-
based GSH probe, FreSHtracer, which reversibly reacts with GSH
accompanied by spectral shifts in UV absorption (520 to 430 nm)
and
fluorescence emission (F580 to F510), allowing ratiometric
measurement of GSH in live cells [5]. Here, we monitored the
changes in GSH levels in HDFs during senescence and evaluated
the effect of antioxidants on GSH levels by measuring the F510/F580
ratio (FR).
oxygen species (ROS) production has a role in inducing
senescence of human dermal
fibroblasts (HDFs) via the activation
of DNA damage and redox signaling pathways [1]. Unfortunately,
ROS-reducing antioxidant agents are only marginally effective in
delaying or preventing cellular senescence, even at a high
concentration [2,3]. We evaluated endogenous antioxidant
systems to better understand these
findings. Glutathione (GSH)
is the most abundant thiol-containing tripeptide that plays a
major role in eliminating H2O2 through a GSH peroxidase-
catalyzed reactions [4]. Moreover, GSH reduces oxidized proteins
through glutathionylation, thereby regulating the intensity and
duration of redox signaling. Thus, intracellular GSH level are
critical for maintaining redox homeostasis. Measuring GSH level
in live cells is challenging owing to the irreversible reaction of
fluorescent probes with GSH. We developed a new coumarin-
based GSH probe, FreSHtracer, which reversibly reacts with GSH
accompanied by spectral shifts in UV absorption (520 to 430 nm)
and
fluorescence emission (F580 to F510), allowing ratiometric
measurement of GSH in live cells [5]. Here, we monitored the
changes in GSH levels in HDFs during senescence and evaluated
the effect of antioxidants on GSH levels by measuring the F510/F580
ratio (FR).